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[目的 ]寻找棘阿米巴角膜炎快速诊断和棘阿米巴快速鉴定的方法。 [方法 ]10 %氢氧化钾 (KOH)湿封片镜检、棘阿米巴培养、倒置显微镜观察、病理切片H .E .染色和SPA染色检查。 [结果 ]角膜刮片及手术切除的角膜材料 ,经 10 %氢氧化钾湿封片镜检 ,前者检出 7例棘阿米巴角膜炎病例 ,后者确诊 5例 ;手术切除的角膜材料经培养 ,分离出 6株棘阿米巴 ;应用倒置显微镜直接观察 ,检出棘阿米巴的包囊、滋养体和棘刺。 [结论 ]应用 10 %KOH湿封片镜检可对棘阿米巴角膜炎作出快速诊断 ;通过倒置显微镜直接观察也可对棘阿米巴角膜炎在 2 0h内作出诊断 ;倒置显微镜可直接观察和鉴定棘阿米巴 ,方法简便、无污染、快速及实用。
[Objective] To find the rapid diagnosis of Acanthamoeba keratitis and the rapid identification of Acanthamoeba. [Method] Microscopy of 10% potassium hydroxide (KOH) wet packing, culture of Acanthamoeba bombillae, observation with inverted microscope, histological examination of H.E. staining and SPA staining. [Results] Corneal scraping and resection of corneal material were examined microscopically with 10% potassium hydroxide wet pack. The former detected 7 cases of Acanthamoeba keratitis, the latter confirmed 5 cases. The surgically removed corneal material Six strains of Acanthamoeba were isolated and cultured. The epithelium, trophozoites and spines of Acanthamoeba were observed by inverted microscopy. [Conclusion] Rapid detection of Acanthamoeba keratitis by 10% KOH wet endoscopy can be used to diagnose Acanthamoeba keratitis directly. Inverted microscope can also be used to make direct diagnosis of Acanthamoeba keratitis in 20 h. And identification of Acanthamoeba, the method is simple, pollution-free, fast and practical.