论文部分内容阅读
以无花瓣油菜APL01与正常有花瓣品种M083杂交的BC1F1为基础群体,利用RAPD、SSR和SRAP技术获得251个分子标记,包括219个SRAP、25个SSR和7个RAPD标记,构建了由19个连锁群组成的分子标记遗传图谱,根据共同的分子标记,建立该图谱与甘蓝型油菜高密度图谱的对应关系。利用WinQTLCart2.0软件对无花瓣性状进行QTL扫描,获得4个与无花瓣性状相关的QTL,QAP5位于N5连锁群的A0226Bb152～m31e40b区间,解释花瓣度表型变异的3.71%;QAP6位于N6连锁群的m25e7～OPY9区间,解释花瓣度表型变异的3.02%;QAP8位于N8连锁群的A0226Gb468～m29e20区间,解释花瓣度表型变异的30.94%;QAP15位于N15连锁群的m21e4b～A0225Bb201区间,解释花瓣度表型变异的21.96%。QAP8和QAP15为2个主效QTL,可用于无花瓣性状的标记辅助选择,QAP5和QAP6为修饰基因位点。
A total of 251 molecular markers, including 219 SRAPs, 25 SSRs and 7 RAPD markers, were obtained from RAPD, SSR and SRAP based on BC1F1, a hybrid between APL01 and M083, Linkage group consisting of molecular marker genetic map, according to the common molecular markers, the establishment of the map and the relationship between the high-density map of Brassica napus. Four QTLs associated with petal-shaped traits were obtained by QTL mapping of the petal-shaped trait using QTLs WinQTLCart2.0. QAP5 was located in the interval of A0226Bb152 ~ m31e40b in the N5 linkage group, accounting for 3.71% of the variance of the petal-shaped phenotype. QAP6 was located in the N6 linkage group QAP8 was located in the interval of A0226Gb468 ~ m29e20 in the N8 linkage group, explaining 30.94% of the variance of the petal degree phenotype; QAP15 was located in the interval of m21e4b ~ A0225Bb201 of the N15 linkage group, and explained the petal Degree of phenotypic variation of 21.96%. QAP8 and QAP15 are two major-effect QTLs, which can be used for marker-assisted selection of petal-shaped traits. QAP5 and QAP6 are modified loci.