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目的:观察中药复方对酒精性肝损害早期阶段阻断作用。方法:将纯系雄性Wistar大鼠50只按体质量随机均分为正常组、模型7d组、模型30d组、中药7d组、中药30d组4组。以正常组和模型组为对照,采用白酒灌胃造模,同时给予中药复方,剂量为21g.kg-1.d-1,12周后,各组大鼠肝脏取材,应用光镜观察大鼠肝脏组织;测定各组大鼠乙醇脱氢酶(ADH)、谷胱甘肽硫转移酶(GST)活性水平及还原型辅酶Ⅰ(NADH)/氧化型辅酶(NAD+);测定各组大鼠肝功能指标。结果:各组大鼠ADH、GST活性水平及NADH/NAD+的测定结果显示,模型7d组与模型30d组较正常组均显著降低(P<0.05),各中药组无明显降低,与同期模型组相比有显著性差异(P<0.05);NADHNAD+模型组较正常组均显著升高(P<0.05),各中药组无明显升高,与各模型组相比有显著性差异(P<0.05)。组织形态学各模型组肝组织损伤严重,各中药组肝组织损伤显著减轻。肝功能各中药组明显优于各模型组。结论:该中药复方较为有效地阻断了酒精对肝脏的损害。
Objective: To observe the blocking effect of traditional Chinese medicine compound on the early phase of alcoholic liver injury. Methods: Fifty pure male Wistar rats were randomly divided into normal group, model group 7d group, model group 30d group, traditional Chinese medicine group 7d group and Chinese traditional medicine group 30d group. The normal group and the model group as the control, the use of white wine gavage modeling, while giving traditional Chinese medicine compound, the dose was 21g.kg-1.d-1, 12 weeks after the liver of each group were drawn, the application of light microscopy rats The levels of ADH, GST and NADH / NAD + of rats in each group were measured. The levels of hepatic Functional indicators. Results: The levels of ADH, GST and NADH / NAD + in each group were significantly lower than those in the normal group (P <0.05), but no significant difference between the model group and the control group (P <0.05); NADH NAD + model group were significantly higher than the normal group (P <0.05), the Chinese medicine group was not significantly increased, compared with the model group was significantly different (P < P <0.05). Histopathology The liver tissue of each model group was severely damaged, and the liver tissue damage of each TCM group was significantly reduced. The liver function of each TCM group was significantly better than the model group. Conclusion: The Chinese medicine compound is more effective in blocking the damage of alcohol on the liver.