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用ＨＢｓＡｇＲＰＨＡ做为模式，选用羊、火鸡、鹅、人Ｏ型血球为载体，醛化后分成致敏和不致敏两种，与异嗜性抗体、中国生物制品药品检定所的参比品等作凝集试验。结果表明，无论特异性还是灵敏度都以人Ｏ型血球为最佳。在此基础上，设计了五种醛化方法处理人Ｏ型血球，共得出１８个醛化样品，经７３种不同的比较试验，筛选出最佳醛化方法为改良丙酮醛──甲醛法，采用该法醛化后血球为褐色，阴性血清和空白对照背景清晰，血球沉集点致密，下滑程度好。致敏抗—ＨＢｓＭｃＡｂ后，使ＨＢｓＡｇ灵敏度达到１～２ｎｇ／ｍｌ。为制备高质量的血凝试剂提供了最佳载体。
Using HBsAgRPHA as the model, sheep, turkey, goose and human O-type blood cells were selected as carriers. After being hydroformylated, the cells were divided into sensitized and non-sensitized, and compared with heterophile antibodies and reference materials of China Biologics and Drug Administration Agglutination test. The results show that no matter the specificity or sensitivity of human O-type blood cells are the best. On this basis, five kinds of hydroformylation methods were designed to process human O-type blood cells, and a total of 18 samples of hydroformylation were obtained. Based on 73 different comparison tests, the best method of hydroformylation was selected as modified acetone- , Using the method after the blood cell is brown, the negative serum and blank control clear background, blood sedimentation point density, a good degree of glide. Sensitized anti-HBsMcAb, the sensitivity of HBsAg reached 1 ~ 2ng / ml. For the preparation of high quality hemagglutination reagent provides the best carrier.