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人体感染出血热病毒后引起的出血热肾综合征,是一种急性间质性肾病,其主要病理生理所见为血管渗透性增加。为了研究汉坦病毒是否在血管内皮细胞中增殖,作者用源自新鲜人脐带静脉的原抗内皮细胞培养及一人脐带静脉内皮细胞传代细胞系(CRL1730)进行本文研究。原代内皮细胞维持在McCoy’s 5A培养液(含20%胎牛血清、25mM HEPES、2.5mM胰岛素及2mM L-谷氨酰胺;CRL1730细胞株每5天传代一次,以1:3分种子覆复盖有人纤维结合素(10μg/cm~2)的培养瓶内,维持在含10%胎牛血清,100μg/ml粗制肝素结合的生长因子
Hemorrhagic fever with renal syndrome caused by infection of the human body with hemorrhagic fever virus is an acute interstitial nephropathy whose vascular pathology shows increased vascular permeability. In order to investigate whether Hantaan virus can proliferate in vascular endothelial cells, we used the original anti-endothelial cell line derived from fresh human umbilical vein and the human umbilical vein endothelial cell line (CRL1730) for this study. Primary endothelial cells were maintained in McCoy’s 5A medium (20% fetal bovine serum, 25 mM HEPES, 2.5 mM insulin and 2 mM L-glutamine; CRL1730 cell lines were passaged every 5 days with 1: 3 seed covers In a culture flask containing fibronectin (10 μg / cm 2), the growth factor was maintained in 10% fetal bovine serum, 100 μg / ml crude heparin-bound growth factor