论文部分内容阅读
以黄芩单细胞克隆愈伤组织为试材,采用聚丙烯酰胺凝胶电泳(PAGE)法,通过分析酯酶同工酶(EST)和过氧化物同工酶(POD)的酶带,研究黄芩单细胞克隆愈伤组织与亲本愈伤组织的遗传特性与品系差异。结果表明:黄芩不同品系单细胞克隆愈伤组织EST谱带间具3个带区特征,最多谱带数品系为4条,最少谱带数品系为3条,其中1号、2号、3号谱带为黄芩单细胞克隆愈伤组织的特征谱带;POD谱带间具4个带区特征,其中1号、3号和7号酶带为特征谱带,EST和POD谱带的有无、宽窄、颜色深浅,相对迁移率大小均不尽相同。黄芩单细胞克隆愈伤组织与亲本细胞愈伤组织既具有亲缘特征又具有品系差异,以期为单细胞克隆品系的遗传特性和品系鉴别提供理论参考。
The single cell clone callus of Scutellaria baicalensis Georgi was used as experimental material, and the enzyme bands of esterase isozyme (EST) and peroxidase isoenzyme (POD) were analyzed by polyacrylamide gel electrophoresis (PAGE) Genetic Characteristics and Lineage Differences of Single Cell Clonal Callus and Parental Callus. The results showed that there were three bands between single band clone callus of Scutellaria baicalensis with the most bands, the least number of bands was 4 and the least number of bands was 3, of which 1, 2, 3 The bands were characteristic bands of single cell clone callus of Scutellaria baicalensis Georgi. There were 4 bands between POD bands, among which bands 1, 3 and 7 were the characteristic bands, and the bands of EST and POD were , Width, color depth, relative mobility are not the same size. The single cell clone callus and the parental cell callus of Scutellaria baicalensis have both genetic and strain differences in order to provide a theoretical reference for the genetic characterization and strain identification of a single cell clone.