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本文采用悬浮式组织培养法用光镜及扫描电镜观察了人体直肠癌细胞系HR8345的体外组织侵袭过程。结果表明,该法可成功显示癌细胞的侵袭性,培养第二天后,癌细胞即以伪足贴附的形式粘附于大鼠心肌表面,然后呈条索状沿着肌纤维间隙向组织深部侵润。侵袭深部的瘤细胞与贴附在组织表面的瘤细啦具有不同的形态特征。本文对瘤细胞体外侵袭机制进行了简要讨论。
In this paper, the suspension tissue culture method using light microscopy and scanning electron microscopy of human rectal cancer cell line HR8345 in vitro tissue invasion process. The results showed that the method can successfully show the invasiveness of cancer cells. After the second day of culture, the cancer cells adhered to the surface of rat myocardium in the form of pseudopod attachment and then appeared in the form of cords along the interstices of muscle fibers into the deep tissue invasion Run. Deep invasion of tumor cells attached to the surface of the tumor with fine friends have different morphological characteristics. In this paper, the in vitro invasion mechanism of tumor cells is briefly discussed.