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J链是一种免疫球蛋白(Ig)多肽,位于细胞核周围、内浆网和高尔基小体内,浆细胞、淋巴细胞和骨髓瘤细胞浆内均有J链存在,核内J链斑点与核仁相关,J链具有促使Ig聚合、分泌、结合、承担免疫应答、标志B细胞恶性肿瘤等多种作用。作者就核仁相关的J链作系统研究并评价其临床意义。受检病例有多发性骨髓瘤(MM)20例,冒烟型MM(SMM)4例,特发性本周氏蛋白尿1例和原发性巨球蛋白血症(PMG)6例。取胸骨骨髓,制备光镜和细胞免疫化学检验标本,分别采用免疫荧光法、PAP法和抗J链抗血清免疫染色法检测各项数值:A.May-Grunwald Giemsa染色计数骨髓浆细胞%;B、500个有核细胞中浆细胞%;C、细胞涂片用FITC和TRITC免疫荧光染色测定500个单克隆浆细胞中J链阳性(J+)细胞%;D、PAP法测定500个
J chain is an immunoglobulin (Ig) polypeptide, located in the nucleus, endoplasmic reticulum and Golgi small bodies, plasma cells, lymphocytes and myeloid cytoplasm are J-chain exists in the nuclear J chain spots and nucleoli Related, J chain has to promote Ig aggregation, secretion, binding, bear the immune response, marking a variety of B cell malignancies. The authors make a systematic study of the nucleolar associated J chain and evaluate its clinical significance. Twenty patients with multiple myeloma (MM), 4 smoked MM (SMM), 1 idiopathic peripheral urogenital proteinuria, and 6 patients with primary macroglobulinemia (PMG) were examined. Immuno-fluorescence, PAP and anti-J-chain antiserum immunostaining were used to detect the value of each specimen: A.May-Grunwald Giemsa stain count% of bone marrow plasma cells; B , 500 nucleated cells in plasma cells%; C, cell smears FITC and TRITC immunofluorescent staining 500 monoclonal clones J chain positive (J +) cells%; D, PAP method for the determination of 500