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对传代细胞系(CCL)培养物制备病毒疫苗的认可为疫苗制备的新技术开辟了道路。CCL的大规模培养大大促进了病毒疫苗的安全性和稳定性。有关残余细胞DNA及其致肿瘤可能性的实验研究显然表明该物质不可能诱发肿瘤。存在于成品中的微量细胞DNA以及疫苗制备过程对DNA的完整性和生物学活性的损害作用,促使人们将残余细胞DNA看成是无实际意义的问题。没有证据表明在成品中限定残余细胞DNA的浓度是有科学根据的。
Recognition of the production of viral vaccines in subcultured cell line (CCL) cultures opens the way for new technologies in vaccine preparation. Large-scale cultivation of CCL has greatly promoted the safety and stability of the virus vaccine. Experimental studies on residual cellular DNA and its potential for causing tumors have clearly shown that it is unlikely that this substance will induce tumors. The presence of trace amounts of cellular DNA in the finished product, as well as the detrimental effects of vaccine preparation on the integrity and biological activity of DNA, has prompted one to see residual DNA as meaningless. There is no evidence that the concentration of residual cellular DNA in the finished product is scientifically justified.