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目的建立高效液相谱对骨力片中淫羊藿苷含量测定的方法,为制剂的质量控制提供依据。方法以淫羊藿苷为含量测定指标,色谱柱Diamonsil C18(250 mm×4.6 mm×5μm);流动相:乙腈-水(25∶75),流速:1.0 ml/min,检测波长:270 nm。结果淫羊藿苷的回归方程:y=1 259.462 5x-1.051 2,r=1.000 0,加标回收率为97.7%,RSD为0.74%。结论该方法具有样品处理简便、测定周期短、灵敏度高、重现性好、专属性强的特点,可以有效地控制骨力片的质量。
OBJECTIVE To establish a HPLC method for the determination of icariin in Bone slices and provide the basis for the quality control of the preparation. Methods The content of icariin was determined. The column was Diamonsil C18 (250 mm × 4.6 mm × 5 μm). The mobile phase was acetonitrile-water (25:75), the flow rate was 1.0 ml / min and the detection wavelength was 270 nm. Results Icariin regression equation: y = 1 259.462 5x-1.051 2, r = 1.000 0, the standard recovery was 97.7%, RSD was 0.74%. Conclusion The method has the characteristics of simple sample processing, short determination period, high sensitivity, good reproducibility and strong specificity, which can effectively control the quality of bone slices.