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本课题包括：①对机会致病寄生虫，包括弓形虫、卡氏肺孢子虫及隐孢子虫动物模型的建立，虫体的分离及提纯；②弓形虫感染的循环抗原（ＣＡ）和抗体（ＩｇＧ、ＩｇＭ），用竞争抑制酶联免疫吸附试验（ＩＥＬＩＳＡ）、金葡菌Ａ蛋白酶联免疫吸附试验（ＳＰＡＥＬＩＳＡ）、直接捕获法酶联免疫吸附试验（ＤＥＬＩＳＡ）进行检测；③用地高辛标记探针及聚合酶链反应（ＰＣＲ）检测核酸，自由引物ＰＣＲ鉴别弓形虫株以及生物特性；④用流式细胞仪对卡氏肺孢子虫及弓形虫的ＤＮＡ、ＲＮＡ及蛋白质含量的动力学进行了分析；⑤用激光微束系统对含８个子孢子成熟包囊施行手术，切除４个或２个子孢子后，包囊仍具有感染力。
The topics include: (1) Establishment of animal models of pathogenic parasites, including Toxoplasma gondii, Pneumocystis carinii and Cryptosporidium parvum, isolation and purification of parasites; (2) Cyclic antigen (CA) and antibodies against Toxoplasma gondii infection IgG, IgM) were detected by enzyme-linked immunosorbent assay (ELISA), SPA-ELISA and direct ELISA (DELISA) (3) Digoxigenin-labeled probe and polymerase chain reaction (PCR) were used to detect the nucleic acid, and PCR was used to identify the Toxoplasma gondii strain and its biological characteristics; ④The DNA, RNA and protein of Pneumocystis carinii and Toxoplasma gondii were analyzed by flow cytometry Content kinetics were analyzed; ⑤ laser microbeam system containing 8 sporozoites mature cysts surgery, removal of 4 or 2 sporozoites, cysts still have the appeal.