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目的研究野牡丹提取液的抗氧化活性。方法采用二苯代苦味肼基自由基(DPPH?)和铁离子还原/抗氧化能力(FRAP)测定法,测定其抗氧化活性,并将其与芦丁和维生素C(Vc)的抗氧化能力进行比较研究。结果野牡丹提取液具有较强的抗氧化能力,对DPPH自由基有较好的清除率,半数清除浓度(IC50)为23μg/mL;对Fe3+也具有较好的还原能力,0.5 mol/L FeSO4当量对应野牡丹提取液质量浓度为55μg/mL。结论野牡丹的抗氧化能力与芦丁相近,低于Vc。野牡丹的抗氧化活性在一定浓度范围内与黄酮的含量有较好的相关性。
Objective To study the antioxidant activity of wild peony extract. Methods Antioxidant activity of dibromobenzylidenebromide (DPPH?) And ferric ion reduction / antioxidant (FRAP) assay were measured and their antioxidant activities were compared with that of rutin and vitamin C (Vc) Make a comparative study. The results showed that the extract of wild Moutan has strong anti-oxidative capacity, good DPPH radical scavenging activity, the half-value clearance concentration (IC50) of 23μg / mL; Fe3 + also has good reducing power, 0.5mol / L FeSO4 Equivalent to wild Moutan extract mass concentration of 55μg / mL. Conclusions The antioxidant capacity of wild peony is similar to that of rutin and lower than Vc. The antioxidant activity of wild peony has a good correlation with flavonoid content in a certain concentration range.