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目的 研究常德市乙型肝炎病毒 (HBV )基因型。 方法 随机选择HBsAg阳性携带者 65例 ,慢性乙型肝炎患者 5例。应用FQ -PCR方法检测血清HBVDNA含量。采用微板核酸杂交 -ELISA技术进行血清HBVA -F6种基因分型。 结果 43例HBVDNA定量阳性血中B、D混合型占 5 1.2 % ( 2 2例 ) ,B型占 2 7.9% ( 12例 ) ,C型占 9.3 % ( 4例 ) ,C、D混合型占 4.7% ( 2例 ) ,B、C混合型占 2 .3 % ( 1例 ) ,未分型者 2例 ,未发现A型、单纯D型、E型和F型。 结论 常德市HBV基因型以B、D混合型为主 ,次为B型、C型 ,C、D混合型及B、C混合型。
Objective To study the genotype of Hepatitis B virus (HBV) in Changde City. Methods 65 cases of HBsAg positive carriers and 5 cases of chronic hepatitis B were randomly selected. Serum HBVDNA content was detected by FQ-PCR. Serum HBVA-F6 genotyping was performed using microplate nucleic acid-ELISA. Results The positive rate of HBV DNA in 43 cases of HBVDNA was 52.2% (22 cases), B (7.9%) (12 cases), C (9.3%), and C and D 4.7% (2 cases), B and C mixed accounted for 2.3% (1 case), 2 cases of undifferentiated type. No A type, simple D type, E type and F type were found. Conclusion The genotypes of HBV in Changde City are mainly B and D, followed by B, C, C, D and B, C.