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目的:探究Smad核内相关蛋白1(Smad nuclear interacting protein 1,SNIP1)在正常宫颈上皮组织及宫颈鳞状细胞癌(宫颈鳞癌)组织中的表达及其意义。方法:免疫组织化学方法及实时荧光定量PCR(Real-time PCR)技术检测SNIP1在23例正常宫颈组织(正常宫颈组)、25例早期宫颈鳞癌组织(Ⅰa~Ⅱa期)(早期宫颈鳞癌组)和45例晚期宫颈鳞癌组织(Ⅱb~Ⅲb期)(晚期宫颈鳞癌组)中的表达。结果:免疫组织化学方法显示SNIP1蛋白在正常宫颈组、早期宫颈鳞癌组及晚期宫颈鳞癌组中胞质强阳性表达率分别为0,36.0%和73.3%;胞核强阳性表达率分别为17.4%,44.0%和71.1%,且三组间胞质与胞核的表达差异均具有统计学意义(P<0.01)。在伴有淋巴结转移的宫颈鳞癌病例中,SNIP1在胞质、胞核中的强阳性率分别高于不伴淋巴结转移病例,且差异有统计学意义(P<0.001)。Real-time PCR技术检测显示SNIP1 mRNA在正常宫颈组、早期宫颈鳞癌组及晚期宫颈鳞癌组中表达呈递增趋势,除了正常宫颈组与早期宫颈鳞癌组间差异无统计学意义外,其余各组间差异有统计学意义(P<0.05)。结论:SNIP1可能参与调控宫颈鳞癌的进展及侵袭转移过程的发生,并有望成为宫颈鳞癌侵袭转移情况的预测因子。
Objective: To investigate the expression and significance of Smad nuclear interacting protein 1 (SNIP1) in normal cervical epithelial tissue and cervical squamous cell carcinoma (cervical squamous cell carcinoma). Methods: Immunohistochemistry and Real-time PCR were used to detect the expression of SNIP1 in 23 cases of normal cervical tissue (normal cervical group), 25 cases of early cervical squamous cell carcinoma (Ⅰa ~ Ⅱa) (early cervical squamous cell carcinoma ) And 45 cases of advanced cervical squamous cell carcinoma (Ⅱb ~ Ⅲb) (advanced cervical squamous cell carcinoma). Results: Immunohistochemistry showed that strong positive cytoplasmic expression of SNIP1 protein in normal cervical group, early cervical squamous cell carcinoma group and advanced cervical squamous cell carcinoma group were 0, 36.0% and 73.3%, respectively. The strong positive expression rate of nuclei were 17.4%, 44.0% and 71.1%, respectively. There were significant differences in the expression of cytoplasm and nucleus between the three groups (P <0.01). In cases of cervical squamous cell carcinoma with lymph node metastasis, the strong positive rates of SNIP1 in cytoplasm and nucleus were significantly higher than those without lymph node metastasis (P <0.001). Real-time PCR showed that the expression of SNIP1 mRNA in normal cervix, early cervical squamous cell carcinoma and advanced cervical squamous cell carcinoma showed an increasing trend, except for the normal cervical group and early cervical squamous cell carcinoma was no significant difference between the groups, the rest The difference between each group was statistically significant (P <0.05). Conclusion: SNIP1 may be involved in the regulation of cervical squamous cell carcinoma progression and invasion and metastasis, and is expected to become a predictor of cervical cancer invasion and metastasis.