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目的:观察柴胡渗湿汤对哮喘大鼠血清中IL-5及IL-13含量的影响,探讨柴胡渗湿汤治疗哮喘的作用机制。方法:将84只雄性Wi star大鼠按随机数字表法分为正常对照组、模型对照组、地塞米松组、定喘汤组、柴胡渗湿汤低剂量组、柴胡渗湿汤中剂量组、柴胡渗湿汤高剂量组,每组12只,采用卵蛋白制作大鼠哮喘模型,予相应药物干预。用酶联免疫吸附试验(ELISA)法检测各组大鼠血清中IL-5及IL-13水平。实验数据采用SPSS11.5统计软件进行分析。结果:实验后各组死亡率比较均无差异,P>0.05;模型对照组与正常对照组血清中IL-5及IL-13含量水平有显著差异,表明大鼠哮喘模型存在着血清IL-5及IL-13含量异常增高的病理状态;与模型对照组相比较,各治疗组血清中IL-5及IL-13的含量明显降低,其中尤以地塞米松组、柴胡渗湿汤中剂量组和柴胡渗湿汤高剂量组的作用更明显。结论:柴胡渗湿汤治疗哮喘的作用机制与降低IL-5及IL-13的水平有关;上述作用具有一定的量效关系,但并不因给药剂量的增加而增加。
Objective: To observe the effect of Chaihu Mushi Decoction on the serum levels of IL-5 and IL-13 in asthmatic rats, and to explore the mechanism of Chaihu Mushi Decoction in treating asthma. Methods: Eighty-four male Wistar rats were randomly divided into normal control group, model control group, dexamethasone group, Dingchutang group, Bupleurum osmanthus decoction low dose group, Bupleurum osmotic wet soup Dose group, Bupleurum and dampness soup high dose group, 12 rats in each group. The asthma model was made by using ovalbumin and corresponding drug intervention was given. Serum levels of IL-5 and IL-13 were detected by enzyme-linked immunosorbent assay (ELISA). Experimental data using SPSS11.5 statistical software for analysis. Results: After the experiment, there was no significant difference in the mortality among the groups (P> 0.05). The levels of IL-5 and IL-13 in the serum of the model control group and the normal control group were significantly different, indicating that the serum IL-5 And IL-13 levels were abnormally increased. Compared with the model control group, the levels of IL-5 and IL-13 in the serum of each treatment group were significantly decreased, especially in the dexamethasone group, Group and Bupleurum wet soup high-dose group more obvious effect. CONCLUSION: The mechanism of Bupleurum Penetration Decoction for the treatment of asthma is related to decreasing the levels of IL-5 and IL-13. The above-mentioned effects have some dose-effect relationship, but not increase with the increase of dose.