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分子生物学的最新进展是发展了“重组DNA”这项新技术。目前已成功地利用这一新技术生产生长抑素、人生长激素和人胰岛索。本文比较了各种来源胰岛素的化学和免疫特性。猪和牛胰岛素新鲜、冰冻的胰脏以酸性乙醇(pH1～3)提取。提取物经真空浓缩、脱脂、盐析和再溶解后,用等电点法(pH5.3～5.4)沉淀。用锌结晶形成具有生物活性的产物。用凝胶层析法和离子交换层析法进一步纯化得到纯度高于99%的胰岛素。剩余物中含有
The latest advances in molecular biology are the development of a new technique called “recombinant DNA.” So far, this new technology has been successfully used to produce somatostatin, human growth hormone and human islet. This article compares the chemical and immunological properties of various sources of insulin. Porcine and bovine insulin Fresh, frozen pancreas is extracted with acidic ethanol (pH 1-3). The extract was concentrated in vacuo, degreased, salted out and redissolved and then resolved by isoelectric point (pH5.3 ~ 5.4). Crystallization with zinc forms a biologically active product. Further purification by gel chromatography and ion exchange chromatography gives insulin> 99% purity. Contained in the residue