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目的 探讨线粒体 Ｄ Ｎ Ａ （ｍ ｔ Ｄ Ｎ Ａ ）相对含量与衰老的关系。方法 采用定量 Ｓｏｕｔｈｅｒｎ 杂交方法测定 ｍ ｔ Ｄ Ｎ Ａ 的相对含量。结果 大鼠大脑组织中老年组 ｍ ｔ Ｄ Ｎ Ａ 的相对含量明显高于其他组（ Ｐ＜ ００１），而幼年与成年组相比无明显差异（ Ｐ ＞００５）。结论 老年组大鼠大脑组织中 ｍ ｔ Ｄ Ｎ Ａ 的含量是增多的，而这种与年龄相关 ｍ ｔ Ｄ Ｎ Ａ 含量的变化可能始于成年以后。
Objective To investigate the relationship between mitochondrial D N A (m t D N A) content and senescence. Methods The relative content of m t D N A was determined by quantitative Southern hybridization. Results Compared with other groups, the relative content of m t D N A in the aged group was significantly higher than that in other groups (P <001). There was no significant difference between the young group and the adult group (P> 005). Conclusions The content of m t D N A in the brain tissue of rats in the aged group is increased, while the age-related content of m t D N A may begin to change after adulthood.