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5. 华山松SRAP引物的筛选与验证

华山松SRAP引物的筛选与验证

来源 :西南林业大学学报 | 被引量 : 0次 | 上传用户：siyu321

【摘 要】

：

为筛选华山松SRAP遗传多样性引物,以华山松无性系种子园6个种源的30株优良无性系幼嫩无病害针叶为实验材料,基于SRAP标记技术,选用10条正向引物、10条反向引物,随机组合成100

【作 者】

：

叶鹏 瞿绍宏 李云芳 罗正平 董云祥 刘成 董章宏 鲁华 辛培尧 

【机 构】

：

西南林业大学西南山地森林资源保育与利用教育部重点实验室,云南昆明,650233云龙县漕涧林场,云南大理,672711;楚雄市紫溪山省级自然保护区管理局,云南楚雄,675000;

【出 处】

：

西南林业大学学报

【发表日期】

：

2020年1期

【关键词】

：

华山松 SRAP 引物筛选 验证 

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论文部分内容阅读

为筛选华山松SRAP遗传多样性引物,以华山松无性系种子园6个种源的30株优良无性系幼嫩无病害针叶为实验材料,基于SRAP标记技术,选用10条正向引物、10条反向引物,随机组合成100个SRAP引物组合,对华山松DNA进行PCR扩增.结果表明:实验共筛选获得15对多态性较为丰富并且具有可重复性的有效引物;15对引物共扩增出3767条DNA条带,其中,多态性条带有2448条,占总条带数的64.99％,平均多态性比率为64.28％.所得15对SRAP引物适合作为华山松遗传多样性分析.

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