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目的 观察运动对心肌和骨骼肌细胞应激激活蛋白激酶 (JNK)信号通道活性的影响 ,为运动处方的制定提供分子生物学依据。方法 1 2周龄、体重 2 0 0～ 2 30克的SD大鼠 1 8只 ,随机分为对照组 (n =6)、急性运动组 (n=6)和运动训练组 (n =6)。采用活动平板 ,坡度 1 0 % ,速度 2 0m/min,运动时间 2 0分 /次。急性运动组仅运动 2 0min ;运动训练组 ,每日 1次 ,每周 5次 ,运动三周 ;对照组不参与运动。运用化学发光测定心肌和骨骼肌JNK活性。结果 骨骼肌JNK活性在急性运动组和运动训练组分别是非运动组的 4 .1倍和1 1 .1倍 ,且运动训练组较急性运动组增高 2 .71倍。心肌JNK活性在急性运动组和运动训练组分别是非运动组的 3 .5倍和 2 .1 6倍 ,且急性运动组较运动训练组增高 1 .62倍。结论 运动可以激活心肌和骨骼肌JNK信号转导通道 ,不同的运动时间和频率影响着心肌和骨骼肌JNK的活性程度
Objective To observe the effects of exercise on the activity of cardiac muscle and skeletal muscle cell stress-activated protein kinase (JNK) signaling and to provide a molecular basis for the formulation of exercise prescription. Methods A total of 18 SD rats aged 2 weeks and weighing 200-230 g were randomly divided into control group (n = 6), acute exercise group (n = 6) and exercise training group (n = 6) . Activity plate, slope 10%, speed 20m / min, exercise time 20 points / time. Acute exercise group only exercise 20min; exercise training group, once daily, 5 times a week, exercise for three weeks; control group did not participate in exercise. Determination of JNK activity in myocardium and skeletal muscle using chemiluminescence. Results The activity of JNK in skeletal muscle was 4.1 times and 11.1 times higher in acute exercise group and exercise training group than in non-exercise group, and increased by 2.71 times in exercise training group compared with acute exercise group. Myocardial JNK activity in acute exercise group and exercise training group were 3.5 times and 2.16 times than non-exercise group, and acute exercise group was 1.62 times higher than exercise training group. Conclusion Exercise can activate JNK signal transduction pathway in myocardium and skeletal muscle. Different exercise time and frequency affect the activity of JNK in myocardium and skeletal muscle