据信多核白细胞(PMN)在 ARDS,肺气肿和隐原性纤维素性肺泡炎的发病机理中起重要作用。PMN 通过其反应性氧化中间产物或释放颗粒中的水解酶和细胞蛋白参与肺损伤。为进一步确定中性粒细胞的颗粒成分在肺损伤中的作用,作者检测了中性粒细胞的细胞毒作用,并比较了对人胚肺细胞和内皮细胞的相关性。方法人 PMN 由市售商品提供,通过离心和低渗分解去除血小板和红细胞,将纯化的 PMN 再除去 Polynuclear leukocytes (PMNs) are believed to play an important role in the pathogenesis of ARDS, emphysema and cryptogenic alveolitis. PMNs participate in lung injury through their reactive oxidation intermediates or release of hydrolases and cellular proteins from the granules. To further determine the role of granule components of neutrophils in lung injury, the authors examined the cytotoxic effects of neutrophils and compared their association with human embryonic lung and endothelial cells. Methods Human PMNs were provided by commercial products, platelets and erythrocytes were removed by centrifugation and hypotonic disintegration, and the purified PMN was removed again