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以玉米不同自交系的幼胚为材料 ,用诱导培养基进行培养 ,以获得愈伤组织。结果发现所有自交系幼胚均可诱导出白色或黄白色的愈伤组织 ,但其再生能力差异显著 ,这主要取决于基因型和培养基种类。自交系 792 2、 340幼胚的再生频率高达 85 .5 %和 78%。植株再生途径也有所不同 ,890 2、 Mo17、 4 112和 84 6以器官发生途径为主 ,340、 792 2、92 1、 85 3和 835以体胚发生途径为主。经长期继代的愈伤组织失去再生能力。通过 RAPD方法发现不同愈伤组织的基因组之间存在差异。通过扫描电镜观察发现玉米体胚发生有两种情况 :一是直接诱导产生胚性愈伤组织和胚状体 ;二是经过多次继代获得胚胎发生
The immature embryos of different inbred lines of maize were used as materials and cultured with induction medium to obtain callus. The results showed that white or yellow-white callus was induced in all inbred embryos, but the regeneration ability was significantly different, which mainly depended on the genotype and medium type. Inbred lines 792 2, 340 embryos regeneration frequency as high as 85.5% and 78%. The pathways of plant regeneration are also different. 890 2, Mo17, 4 112 and 84 6 are mainly organogenesis pathways, and 340, 792 2, 92 1, 85 3 and 835 are dominated by somatic embryogenesis. After long-term succession of callus lost regenerative ability. Differences between the genomes of different callus were found by RAPD method. Scanning electron microscopy found that there are two cases of somatic embryogenesis: First, direct induction of embryogenic callus and embryoid body; the second is obtained after multiple generations of embryogenesis