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目的:建立清金得生片中华蟾酥毒基和酯蟾毒配基的含量测定方法。方法:采用Hypersil ODS色谱柱(250mm×4mm,5μm);以乙腈-0.5%磷酸二氢钾溶液(用磷酸调pH至3.2)(45:55)为流动相;检测波长为296nm;柱温35℃。结果:华蟾酥毒基和酯蟾毒配基分别在0.04～1.25μg(r=0.999 9)、0.04～1.24μg(r=0.9998)范围内线性关系良好,加样回收率分别为99.37%(RSD=1.71%),100.20%(RSD=1.37%)。结论:方法简便、结果准确,可作为清金得生片质量控制指标之
OBJECTIVE: To establish a method for the determination of the content of Chinese wolfberry glycogen and ester glycosides in Qingjindesheng Tablets. Methods: Hypersil ODS column (250mm×4mm, 5μm); acetonitrile-0.5% potassium dihydrogen phosphate solution (pH adjusted to 3.2 with phosphoric acid) (45:55) as the mobile phase; detection wavelength was 296nm; column temperature 35 °C. RESULTS: The linear relationship was good in the range of 0.04 to 1.25 μg (r=0.999 9) and 0.04 to 1.24 μg (r=0.9998), respectively. The recoveries of the samples were 99.37% (RSD). =1.71%), 100.20% (RSD = 1.37%). Conclusion: The method is simple, accurate, and can be used as a quality control index for Qingjindesheng tablets.