介绍了应用聚合酶链反应技术检测女性宫颈炎患者沙眼衣原体感染实验方法的建立和临床应用，并应用该方法检测女性非淋菌性宫颈炎１２２例，其中５１例阳性，占４１．８％；淋菌性宫颈炎患者９６例，２９例阳性，占３０．２％。说明该方法特异性、敏感性较高，值得推广应用。 The establishment and clinical application of polymerase chain reaction (PCR) technique to detect Chlamydia trachomatis infection in female patients with cervicitis were introduced. 122 cases of female non-gonococcal cervicitis were detected by this method, of which 51 cases were positive (41.8%). 96 cases of cervicitis patients, 29 cases were positive, accounting for 30.2%. It shows that the method is specific and sensitive, so it is worth popularizing and applying.