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目的 探讨细胞周期调控与癌、抑癌基因在肝细胞癌 (hepatocellularcarcinoma ,HCC)中的表达。 方法提取 2 2例HCC患者肝癌及癌旁组织的mRNA ,逆转录合成含有3 3 p dATP标记的cDNA探针 ,与含有 3170个基因或EST(表达序列标签 )的cDNA微阵列进行杂交。结果 肝癌组织与癌旁组织间共有 136 9个差异表达基因或EST ,其中有 12 1个基因或EST表达差异 >2倍。肝癌组织与癌旁组织比较 ,12 1个基因或EST中表达上调的有88个 ,表达下调的有 33个 ;其中一些基因或EST在HCC中的表达上调尚属首次报道。结论 细胞周期调控与癌、抑癌基因参与了肝癌的发生 ,应用微阵列技术揭示了肝癌的基因表达谱 ,为研究肝癌的细胞周期调控与癌、抑癌基因提供了重要的线索。
Objective To investigate the expression of cell cycle regulators and tumor suppressor genes in hepatocellular carcinoma (HCC). Methods The mRNA of hepatocellular carcinoma and its adjacent tissues was extracted from 22 patients with HCC. CDNA probes containing 3 3 p dATP were reverse transcribed and hybridized with cDNA microarray containing 3170 genes or EST (expressed sequence tag). Results There were 136 9 differentially expressed genes or ESTs between hepatocellular carcinoma and adjacent non-cancerous tissues, of which 12 1 genes or ESTs> 2-fold difference. There were 88 up-regulated and 11 down-regulated genes in 12 1 genes or ESTs, compared with the adjacent normal tissues. The up-regulation of some genes or EST in HCC was the first reported. Conclusion Cell cycle regulation, cancer and tumor suppressor genes are involved in the development of hepatocellular carcinoma. Using microarray technology to reveal the gene expression profile of hepatocellular carcinoma and provide important clues for the study of cell cycle regulation and cancer and tumor suppressor genes in hepatocellular carcinoma.