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目的:探索新型肿瘤坏死因子(TNF)相关的凋亡诱导配体(TRAIL)对乙型肝炎病毒(HBV)感染后的免疫监视效应。方法:应用酶联免疫吸咐(ELISA)和噻唑蓝(MTT)比色法及流式细胞术检测52例HBV感染者和24例健康对照者血清中可溶性TRAIL(sTRAIL)的表达,在转染HBV全基因组并稳定表达病毒抗原的HepG2.2.15肝癌细胞系研究TRAIL在γ鄄干扰素(IFN鄄γ)的作用下对HepG2.2.15细胞的生长抑制效应和凋亡诱导效应。结果:HBV感染者的外周血sTRAIL的表达量明显高于正常对照组(P<0.001)。各组HBV感染者sTRAIL的表达量与丙氨酸氨基转换酶(ALT)的值呈正相关。而细胞学的研究结果显示,在IFN鄄γ的协同作用下,TRAIL能够显著抑制HepG2.2.15细胞的恶性增殖,并能够显著诱导HBV相关肿瘤细胞发生凋亡。结论:TRAIL是参与到HBV感染后所致疾病中的一个重要的免疫监视分子,TRAIL的作用效应极有可能直接影响疾病的预后与转归。
Objective: To explore the immune surveillance effect of a novel tumor necrosis factor (TNF) related apoptosis inducing ligand (TRAIL) on hepatitis B virus (HBV) infection. Methods: Serum levels of soluble TRAIL (sTRAIL) were detected by enzyme-linked immunosorbent assay (ELISA), MTT assay and flow cytometry in 52 HBV infected patients and 24 healthy controls. HepG2.2.15 Hepatocellular Carcinoma Cell Line Stably Expressed with HBV Antigen in Hepatocellular Carcinoma Cell Line HepG2.2.15 Cells were Studied for the Growth Inhibitory Effect and Apoptosis-inducing Effect of TRAIL on HepG2.2.15 Cell Line by γ-interferon (IFN-γ). Results: The expression of sTRAIL in the peripheral blood of HBV infected patients was significantly higher than that of the normal controls (P <0.001). The expression of sTRAIL in HBV infected patients was positively correlated with the value of alanine aminotransferase (ALT). The results of cytology showed that under the synergistic effect of IFN-γ, TRAIL could significantly inhibit the malignant proliferation of HepG2.2.15 cells and induce the apoptosis of HBV-related tumor cells significantly. CONCLUSIONS: TRAIL is an important immune surveillance molecule involved in the disease caused by HBV infection. The effect of TRAIL is likely to directly affect the prognosis and outcome of the disease.