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导言小麦品种有时可根据籽粒的形态特征进行鉴定.但是,许多品种的质量属性难以辨别.电泳便是解决这一问题的一种方法.采用电泳方法进行品种鉴定是以麦胶蛋白的分析为基础的.麦胶蛋白是醇溶蛋白质的一个异质组,大约占小麦种子贮藏蛋白质的一半.电泳分析,首先要用乙醇将麦胶蛋白从种子或面粉中提取出来,并将提取物纯化,然后将提取液置于聚丙烯酰胺凝胶上,电流通过凝胶即可使麦胶蛋白分离,形成一系列条带.在电泳所用的
Introduction Wheat varieties can sometimes be identified based on the morphological characteristics of the grain, but the quality attributes of many varieties are indistinguishable. Electrophoresis is one way to deal with this problem. The use of electrophoresis to identify cultivars is based on the analysis of gliadin Gliadin is a heterogeneous group of prolamin proteins, accounting for about half of the storage of wheat seeds electrophoretic analysis, first of all, the gliadin is extracted from the seeds or flour with ethanol and the extract is purified, The extract was then placed on a polyacrylamide gel and the current passed through the gel to separate the gliadin to form a series of bands.