论文部分内容阅读
目的:比较利维爱和尼尔雌醇对去卵巢大鼠骨质疏松的作用并探讨其作用机制。方法:建立去卵巢大鼠骨质疏松模型,40只雌性大鼠随机分成4组:对照组、去卵巢组、尼尔雌醇组和利维爱组。于去卵巢术后1周分别予以利维爱和尼尔雌醇灌胃,10周后观察各组大鼠体重、骨密度及骨形态学的改变,并通过RIA法检测各组大鼠骨组织微环境中细胞因子IL-6和TNF-α的含量。结果:去卵巢组较对照组体重明显增加(P<0.01),骨密度显著下降(P<0.01),骨组织中IL-6、TNF-α含量均显著增高(P<0.01)。尼尔雌醇组体重明显低于去卵巢组(P<0.01)。两组骨密度均显著高于去卵巢组(P<0.01)。骨组织中IL-6、TNF-α含量均显著低于去卵巢组(P<0.01)。但尼尔雌醇组骨密度仍显著低于对照组(P<0.05)。结论:尼尔雌醇较利维爱能更好地抑制去卵巢大鼠的体重增加,二者均能有效预防去卵巢所致大鼠的骨质疏松,其作用机制可能是二药均能有效地抑制去卵巢大鼠骨组织微环境中细胞因子IL-6和TNF-α的含量而减少骨丢失。
Objective: To compare the effect of Levi and nilestriol on osteoporosis in ovariectomized rats and to explore its mechanism. Methods: Osteoporosis model was established in ovariectomized rats. Forty female rats were randomly divided into 4 groups: control group, ovariectomized group, nilestriol group and levofloxacin group. One week after ovariectomy, the rats were administered with Levi and gavage respectively. After 10 weeks, the body weight, bone mineral density and bone morphological changes of the rats in each group were observed. The bone mineral density Microenvironment of cytokines IL-6 and TNF-α content. Results: The body weight of ovariectomized group was significantly higher than that of control group (P <0.01), and the BMD was significantly decreased (P <0.01). The contents of IL-6 and TNF-α in bone were significantly increased (P <0.01). The body weight of the nilestriol group was significantly lower than that of the ovariectomized group (P <0.01). Bone mineral density was significantly higher in both groups than in ovariectomized group (P <0.01). The contents of IL-6 and TNF-α in bone tissue were significantly lower than those in ovariectomized group (P <0.01). However, the BMD in nilestriol group was still significantly lower than that in control group (P <0.05). CONCLUSION: Nilestriol is more effective than Levocaine in inhibiting the weight gain of ovariectomized rats, both of which can effectively prevent osteoporosis in ovariectomized rats, and its mechanism may be that both drugs are effective To reduce the content of cytokines IL-6 and TNF-α in bone tissue microenvironment of ovariectomized rats to reduce bone loss.