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目的建立测定骨髓炎载药人工骨植入术后患者引流液中万古霉素含量的HPLC方法。方法以去甲万古霉素为内标,采用CAPCELL PAK C_(18)(250 mm×4.6 mm,5μm)色谱柱,流动相:0.05 moL·L~(-1)磷酸二氢钾溶液(调节pH至3.2)-乙腈(91.5:8.5),DAD检测器,检测波长280 nm,流速1 mL·min~(-1),柱温30℃,引流液经高氯酸沉淀蛋白后进样测定。结果万古霉素含量测定方法的线性范围为23.17~695.10 mg·L~(-1),r=0.9999(n=10),平均回收率102.53%,RSD=2.49%,术后第3天引流液中万古霉素浓度达峰值,累积释放量为45%。结论该方法快速简单,重复性好,适用于引流液中万古霉素浓度的检测。
Objective To establish an HPLC method for the determination of vancomycin in patients with osteomyelitis drug-loaded artificial bone graft. Methods Norvancomycin was used as an internal standard and the mobile phase was CAPCELL PAK C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was 0.05 mol L -1 potassium dihydrogen phosphate solution To 3.2) - acetonitrile (91.5: 8.5). The detection wavelength was 280 nm and the flow rate was 1 mL · min ~ (-1). The column temperature was 30 ℃. Results The linear range of determination of vancomycin was 23.17-695.10 mg · L -1, r = 0.9999 (n = 10). The average recovery was 102.53% and RSD was 2.49% Vancomycin peak concentration, the cumulative release of 45%. Conclusion The method is rapid, simple and reproducible. It is suitable for the determination of vancomycin in drainage fluid.