为了解非小细胞肺癌中ｐ５３基因突变的情况，应用改良聚合酶链反应－单链构象多态（ＰＣＲ－ＳＳＣＰ）银染技术检测１７例非小细胞肺癌中ｐ５３基因第５～８外显子的突变情况，有８例存在ｐ５３基因突变，突变率为４７．００％（８／１７），其中鳞癌为５５．５６％（５／９），腺癌为３７．５０％（３／８）。本研究结果表明：非小细胞肺癌中ｐ５３基因突变较为常见；改良后的ＰＣＲ－ＳＳＣＰ银染技术对非小细胞肺癌的基因诊断具有潜在性应用价值 To understand the p53 gene mutation in non-small cell lung cancer, PCR-SSCP silver staining was used to detect exons 5-8 of the p53 gene in 17 cases of non-small cell lung cancer. In the case of mutation, there were 8 cases of p53 mutation, the mutation rate was 47.00% (8/17), among which 55.56% (5/9) of squamous cell carcinoma and 37.50% of adenocarcinoma (3/8) ). The results of this study indicate that p53 gene mutations are more common in non-small cell lung cancer; the improved PCR-SSCP silver staining technique has potential application value in the gene diagnosis of non-small cell lung cancer.