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目的:研究黄芪内生真菌AR-15-1的环二肽类次生代谢产物的成分。方法:内生真菌AR-15-1是采用组织学方法分离自恒山黄芪的根部,真菌的鉴定采用形态学法结合分子生物学法,内生真菌大量发酵采用固体培养的方法,环二肽成分的分离使用柱色谱法,HPLC法结合LH-20羟丙基葡聚糖凝胶色谱进行分离纯化,化合物结构鉴定采用波谱表征分析。结果:从黄芪根部分离到的内生真菌AR-15-1被鉴定为曲霉属Aspergillus sp.,从AR-15-1的固体发酵物中分离到8个环二肽化合物,分别为环(脯-甘)二肽(1),环(脯-亮)二肽(2),环(脯-异亮)二肽(3),环(脯-缬)二肽(4),环(苯丙-甘)二肽(5),环(苯丙-丙)二肽(6),环(苯丙-络)二肽(7)和环(苯丙-脯)二肽(8)。结论:环二肽类化合物1~8均为首次从黄芪内生真菌中分离得到,其中大部分环二肽化合物已被发现是具有很好的生物活性。
Objective: To study the components of the cyclic dipeptide secondary metabolites of AR-15-1. Methods: The endophytic fungus AR-15-1 was isolated from the roots of Astragalus membranaceus by histological method. The morphological and molecular biological methods were used to identify the fungi. The endophytic fungi were fermented in large amount by solid culture method. Separation and purification using column chromatography, HPLC method combined with LH-20 hydroxypropyl Sephadex chromatography, the structure of the compounds were identified by spectral analysis. Results: The endophytic fungus AR-15-1 isolated from the root of Astragalus mongholicus was identified as Aspergillus sp., And eight cyclic dipeptide compounds were isolated from the solid fermentation products of AR-15-1, (2), cyclic (pro-isol) dipeptide (3), cyclic (pro-valine) dipeptide (4), cyclic -glycans) dipeptide (5), cyclic (phenylpropionyl) dipeptide (6), cyclic (phenylpropylene-co) dipeptide (7) and cyclic (phenylpropionate) dipeptide (8). CONCLUSION: Cyclic dipeptides 1 ~ 8 were isolated from the endophytic fungi of Astragalus for the first time. Most of the cyclic dipeptide compounds have been found to have good biological activity.