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为获取栗疫病生物防治的基础信息,本研究根据菌株在PDA培养基上培养7天后的菌丝生长速度、分生孢子形成能力等培养特性,从韩国国立山林科学院树木病理研究室保藏的60个栗疫病菌菌株中筛选了2个弱致病力菌株,进行dsRNA检测、弱致病力菌株和强致病力菌株间的细胞融合试验。结果表明:2个弱致病力菌株(KCP-135和KCP-136)中均检测到了dsRNA,弱毒性菌株KCP-22和其他19个强毒性菌株之间的菌落形成明显的隔离带并沿着隔离带产生分生孢子,没有明显的细胞融合现象,而弱毒性菌株KCP-22和强毒性菌株KCP-9之间的菌落则呈现了显著的细胞融合现象,而且其细胞融合菌株的培养特性和转化dsRNA数量均发生了变异。
In order to obtain the basic information of biological control of chestnut blight, according to the culture characteristics of mycelial growth rate, conidial formation ability and so on after cultured for 7 days in PDA medium, this study collected from 60 trees in the Laboratory of Pathology, Korea National Academy of Forestry Two Cryptopathogenic strains were screened for the weak virulence strains for dsRNA detection, weak fusion virulence strains and virulent strains of cell fusion test. The results showed that colonies between dsRNA, weakly virulent strain KCP-22 and other 19 virulent strains were found to have significant isolates in both weak virulent strains (KCP-135 and KCP-136) Conidia were formed in the isolates, and no obvious cell fusion was observed. However, the colony between the virulent strain KCP-22 and the virulent strain KCP-9 showed significant cell fusion phenomenon. Moreover, the culture characteristics of the cell fusion strains and The number of transformed dsRNA mutated.