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目的探讨电磁场对骨肉瘤细胞系MG63细胞化疗增敏作用的机制。方法以频率为50Hz,强度为0.8mT、1.6mT、3.2mT的电磁场,对具有一定阿霉素浓度的MG63细胞进行磁场干预,采用四甲基偶氮唑蓝(MTT)法检测并确定磁场对骨肉瘤化疗作用最明显的磁场强度,逆转录酶聚合酶链反应技术(RT-PCR)检测在最佳磁场作用下增殖细胞核抗原(PCNA)和SurvivinemRNA的表达以及流式细胞仪检测细胞周期情况。结果MTT显示磁场化疗组(D组)细胞生存率较单纯阿霉素化疗组(B组)下降,且1.6mT作用最明显;RT-PCR结果显示PCNAmRNA、SurvivinemRNA表达下降;流式细胞仪检测D组G2/M期阻滞较B组高,D组G2/M期细胞达(80.20±0.96)%,高于B组的(18.08±1.50)%、单纯磁场组(C组)的(55.11±2.20)%(P<0.05)。结论适当窗口的电磁场作用可以提高肿瘤的化疗效果,为临床化疗提供一个新的辅助措施。
Objective To investigate the mechanism of electromagnetic field on the chemosensitivity of osteosarcoma cell line MG63. Methods The magnetic field of MG63 cells with a certain concentration of doxorubicin was induced by electromagnetic field of frequency 50Hz, intensity 0.8mT, 1.6mT, 3.2mT. The magnetic field was detected by MTT method Osteosarcoma showed the most obvious magnetic field intensity. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of proliferating cell nuclear antigen (PCNA) and Survivine mRNA under the optimal magnetic field and the cell cycle by flow cytometry. Results MTT showed that the cell survival rate in magnetic field chemotherapy group (group D) was lower than that in the chemotherapy group (group B), and the effect of 1.6mT was the most obvious. The expression of PCNA mRNA and Survivine mRNA was decreased by RT-PCR, The G2 / M phase arrest in group D was higher than that in group B, the percentage of G2 / M phase in group D was (80.20 ± 0.96)%, higher than that in group B (18.08 ± 1.50)%, that in group C 2.20)% (P <0.05). Conclusion Appropriate window of the electromagnetic field can improve the chemotherapy effect of the tumor and provide a new auxiliary measure for clinical chemotherapy.