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淋巴细胞亚群分析主要指用单克隆抗体(McAb)和流式细胞计(FCM)进行表面标志的分析。作者就淋巴细胞亚群分析的现状和临床应用,概述如下。 一、淋巴细胞亚群分析的现状 淋巴细胞亚群的分析,首先从T细胞、B细胞的分类开始。T细胞又进一步区分为CD_4细胞、CD_8细胞。详细的分析以CD_4细胞内的亚群和CD_8细胞内的亚群为主体。最近也很少单独使用McAb标记的CD_4或CD_8这种大的亚群标志。淋巴细胞的功能亚群标志分析,主要指体外的功能亚群分析。都是在非常限定的条件下进行的,其结果未必具有普遍意义。主要用标记区别亚群,是否确切或仅是偶然与其功能有关,还很难判定。这些亚群实际上作为功能亚群使用,所以有必要进行深入研究和临床评价。基础和临床研究已发现CD_4和CD_8
Lymphocyte subsets analysis mainly refers to the analysis of surface markers using monoclonal antibodies (McAb) and flow cytometry (FCM). The authors present the status and clinical application of lymphocyte subsets analysis as summarized below. First, the status of lymphocyte subsets Analysis of lymphocyte subsets, first from the T cells, B cell classification began. T cells were further divided into CD 4 cells, CD 8 cells. Detailed analysis of CD_4 cells and CD_8 cells in the sub-group as the main body. It has also been rare to use McAb-labeled large subsets of CD_4 or CD_8 alone. Functional subpopulations of lymphocytes marker analysis, mainly refers to the functional subpopulations in vitro analysis. Are carried out under very limited conditions and the results do not necessarily have universal significance. It is hard to judge whether the subgroups are mainly marked by marks, whether they are exact or just accidentally related to their function. These subpopulations are actually used as functional subsets, so there is a need for further research and clinical evaluation. Basic and clinical studies have found CD_4 and CD_8