论文部分内容阅读
利用高效液相色谱法,同时测定不同产地西红花中的西红花苷-Ⅰ和西红花苷-Ⅱ的含量;采用Agilent Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm),甲醇-水(45∶55,V/V)洗脱,流速为1.0 m L·min-1,检测波长为440 nm,柱温为25℃。方法学考察表明:在上述色谱条件下,西红花苷-Ⅰ和西红花苷-Ⅱ能与杂质完全分离;标准曲线线性关系良好(R2>0.999 0),精密度和稳定性符合分析要求,平均加样回收率分别为99.42%、99.36%,RSD分别为1.48%、1.28%,表明所建立的方法重复性、耐用性、准确度良好。对10批不同产地的西红花药材进行分析,结果显示产自河南南阳、河南许昌、浙江湖州和河北定州的西红花的总苷含量高于其他产地,为西红花药材的质量评价和控制提供了一定的科学依据。
The contents of crocin-I and crocin-II in saffron from different areas were determined simultaneously by HPLC. An Agilent Eclipse XDB-C18 column (250 mm × 4.6 mm, 5 μm) Methanol-water (45:55, V / V) at a flow rate of 1.0 m L · min-1 at a detection wavelength of 440 nm with a column temperature of 25 ° C. Methodological studies showed that under the above chromatographic conditions, crocin-Ⅰ and crocin-II were completely separated from the impurities; the calibration curve had good linearity (R2> 0.999 0), and the precision and stability were in accordance with the analytical requirements The average recoveries were 99.42% and 99.36%, respectively. The RSDs were 1.48% and 1.28%, respectively. The results showed that the established methods were reproducible, robust and accurate. The results showed that the total glycoside content of saffron grown in Nanyang of Henan, Xuchang of Henan, Huzhou of Zhejiang and Dingzhou of Hebei was higher than that of other producing areas. The quality evaluation of saffron medicinal materials And control provides a certain scientific basis.