Raman spectroscopy is widely used in chemical, physical, biological and geological sciences, where it is used for both qualitative and quantitative studies. Because each scattering molecular species has its own characteristic Raman spectrum, the method often is used to simply identify a chemical species. The intensity of the peaks can be used to measure the concentration of the scattering species. Changes in wave number (vibrational frequency or peak position) may be used to study the effects of density, temperature and pressure on a chemical species. When the environment (such as the temperature and pressure) of the system changes, the spatial configuration of atoms is affected by slight adjustments in the covalent bonds. As a result the vibrational frequency and the wavenumber of the Raman spectrum are shifted. By heating or cooling the inclusions, the internal pressure of the inclusions vary accordingly with temperature since the inclusions represent a constant volume system. The objective of the current research is to document the effect of the pressure and temperature on the measured spectral parameters of a given species in a fluid inclusion, and thereby to generate a data set that can be used to measure the total pressure within a fluid inclusion at any given temperature.;The reported experiments here were conducted using a DILOR XY molar laser Raman microprobe on three synthetic CH 4 H 2O fluid inclusions containing 6565ppm, 17192ppm and 49189ppm CH 4. The excitation energy was provided by a 514 nm Ar + laser using a power setting of 100 mW. The spectrometer gratings were set at 1200 grooves/mm resulting in a resolution of ±3cm -1 . An analysis time of 300 seconds was selected to maximize the signal to noise ratio. The slit width was 100 μm. The Raman peak position of methane was measured at different temperatures from 25°C to the final homogenisation temperature of the inclusion at an increment of 25°C degree using a Chaixmeca heating stage attached to the microscope stage of the Raman microprobe. The peak positions of the band of CH 4 in CH 4 H 2O fluid inclusion were measured at different temperatures. The results show that a noticeable change in the peak position occurs at the homogenisation temperature, due to a phase transition for CH 4 vapour to CH 4 liquid at this temperature. The shift of the peak position during this phase transitions is considerably lager than the shift associated with temperature variation, which was noticed also in previous studies (A.V. Sechkarev and N.I. Dvorovenko, 1965). Based on the peak positions, the partial pressure of CH 4 within the inclusion, P CH 4 ( T ), was calculated. The partial pressure of water, P H 2O ( T ), was obtained from the data available in NBS/NRC steam table. The total internal pressure of the inclusion, P total ( T ), at a given temperature is the sum of the P CH 4 ( T ) and P H 2O ( T ) at that temperature. Raman spectroscopy is widely used in chemical, physical, biological and geological sciences, where it is used for both qualitative and geological sciences, where it is used for both qualitative and geological studies. The intensity of the peaks can be used to measure the concentration of the scattering species. Changes in wave number (vibrational frequency or peak position) may be used to study the effects of density, temperature and pressure on a chemical species. such as the temperature and pressure) of the system changes, the spatial configuration of atoms is affected by slight adjustments in the covalent bonds. As a result of the vibrational frequency and the wavenumber of the Raman spectra are shifted. By heating or cooling the inclusions, the internal pressure of the inclusions vary accordingly with temperature since the inclusions represent a constant volume system. The ob jective of the current research is to document the effect of the pressure and temperature on the measured spectral parameters of a given species in a fluid inclusion, and thus to generate a data set that can be used to measure the total pressure within a fluid inclusion at any given temperature. ; The reported experiments here were conducted using a DILOR XY molar laser Raman microprobe on three synthetic CH 4 H 2 O fluid inclusions containing 6565 ppm, 17192 ppm and 49189 ppm CH 4. The excitation energy was provided by a 514 nm Ar + Laser using a power setting of 100 mW. The spectrometer gratings were set at 1200 grooves / mm resulting in a resolution of ± 3 cm -1. An analysis time of 300 seconds was selected to maximize the signal to noise ratio. μm. The Raman peak position of methane was measured at different temperatures from 25 ° C to the final homogenisation temperature of the inclusion at an increment of 25 ° C degree using a Chaixmeca heating stage attached to the microscope stage of the Raman microprobe. The peak positions of the band of CH 4 in CH 4 H 2O fluid inclusion were measured at different temperatures. The results show that a noticeable change in the peak position occurs at the homogenization temperature, due The shift of the peak position during this phase transitions is contributing lager than the shift associated with temperature variation, which was noticed also in previous studies (AV Sechkarev and NI Dvorovenko , Based on the peak positions, the partial pressure of CH 4 within the inclusion, P CH 4 (T), was calculated. The partial pressure of water, PH 2O (T), was obtained from the data available in NBS / NRC steam table. The total internal pressure of the inclusion, P total (T), at a given temperature is the sum of the P CH 4 (T) and PH 2O (T) at that temperatur e.