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目的:探讨地西他滨(decitabine,DCA)和丙戊酸钠(valproic acid,VPA)联用对白血病细胞株U937的促凋亡和分化影响。方法:设立分组如下:对照组,DCA单药A组(1.0μmol/L),DCA单药B组(4.0μmol/L),VPA单药组(2.0 mmol/L),联合用药A组(DCA 1.0μmol/L+VPA 2.0 mmol/L),联合用药B组(DCA 4.0μmol/L+VPA 2.0 mmol/L),作用48 h。应用Annexin V-FITC/PI标记法检测早期凋亡率,流式细胞术检测CD34、CD117 MFI以及CD11b、CD14表达率。结果:联合用药组A、B的凋亡率高于其各自的单药组,差异具有显著统计学意义(P<0.01);联合用药A、B组的CD117和CD34 MFI低于其各自的单药组,差异具有显著统计学意义(P<0.01);联合用药A、B组的CD11b和CD14表达率低于其各自的单药组,差异具有显著统计学意义(P<0.01)。结论:U937细胞中VPA能显著增强DCA的促凋亡分化作用。
Objective: To investigate the effects of decitabine (DCA) and valproic acid (VPA) on the pro-apoptotic and differentiation of leukemia cell line U937. Methods: The following groups were established: control group, DCA single drug group (1.0μmol / L), DCA single drug group B (4.0μmol / L), VPA single drug group (2.0mmol / L) 1.0μmol / L + VPA 2.0 mmol / L) for 48 h in combination group B (4.0μmol / L DCA and 2.0 mmol / L VPA). The rate of early apoptosis was detected by Annexin V-FITC / PI and the expression of CD34, CD117, MFI and CD11b, CD14 were detected by flow cytometry. Results: The apoptosis rates of A and B in combination group were higher than those in single drug group (P <0.01). The MFI of CD117 and CD34 in combination group A and B were lower than their single (P <0.01). The expression rates of CD11b and CD14 in group A and group B were lower than those in group A and group B respectively (P <0.01). The difference was significant (P <0.01). Conclusion: VPA in U937 cells can significantly enhance the pro-apoptotic differentiation of DCA.