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坐骨神经分支选择性损伤 (SNI)模型是一种新型神经病理痛模型。本实验用 SD雄性大鼠 ,分支结扎并切断左侧坐骨神经干的胫神经和腓总神经 ,保留腓肠神经分支 ,右侧仅暴露坐骨神经。术后 1、2、3、4、7、14、2 1和 2 8d,用 RT-PCR的方法对 5 -HT1 A受体 m RNA在腰髓的背角和背根神经节 (DRG)的表达水平进行检测。结果显示 ,5 -HT1 A受体 m RNA在损伤侧腰髓背角内的表达水平于 1d后开始升高 ,7d时达高峰 ,随后逐渐下降 ,但仍高于正常水平。其表达水平在对侧脊髓背角内没有明显变化。在损伤侧 DRG内 ,5 -HT1 A受体 m RNA的表达水平于 1d后开始增高 ,4d时达高峰 ,随后开始下降 ,但仍维持较高的表达水平 ;而损伤对侧 DRG内的 5 -HT1 A受体 m RNA的表达没有变化。上述结果提示 5 -HT1 A受体亚型可能在脊髓及外周伤害性信息的传递和调节中发挥着重要作用 ,本研究的结果为进一步了解 5 -HT1 A受体在神经病理性痛中的作用机制提供了依据。
Sciatic Nerve Branch-selective Injury (SNI) Model is a Novel Neuropathic Pain Model. In this experiment, SD male rats were ligated and cut off the tibial nerve and common peroneal nerve of the left sciatic nerve trunk, the branch of the sural nerve was retained, and only the sciatic nerve was exposed on the right side. At 1, 2, 3, 4, 7, 14, 21, and 28 days after operation, the 5-HT1 A receptor m RNA was detected by RT-PCR in the dorsal horn and dorsal root ganglion (DRG) Expression level for testing. The results showed that the expression level of 5-HT1 A receptor m RNA in lumbar dorsal horn of lumbar spinal cord began to increase after 1 day and peaked at 7 days, then decreased gradually, but still higher than normal level. The expression level in the contralateral spinal cord dorsal horn did not change significantly. The expression level of 5-HT1 A receptor m RNA began to increase in 1d after DRG injury and peaked at 4d, then began to decline, but still maintained a high level of expression. However, the expression of 5 - There was no change in the expression of HT1 A receptor m RNA. The above results suggest that 5-HT1A receptor subtypes may play an important role in the transmission and regulation of nociceptive information in the spinal cord and periphery. The results of this study are to further understand the mechanism of action of 5-HT1A receptor in neuropathic pain Provided the basis.