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目的 :探讨Lac启动子在可溶性抗体Fab表达中的作用。方法 :利用DNA重组的方法对P3 -3G9噬菌体抗体表达载体[1 ] 进行改建 ,通过免疫印迹试验鉴定新的载体P3G9-L是否有可溶性Fab的表达。结果 :1.P3G9-L为单Lac启动子同时驱动Fd段及K链的双顺反子的可溶性Fab段表达载体。 2 .P3G9-L中的Lac启动子的作用是缓和的 ,可溶性Fab表达量较低。结论 :1 Lac启动于可以用于可溶性抗体Fab的表达体系。 2 双Lac启动子完全可由单Lac启动子取代
Objective: To investigate the role of Lac promoter in the Fab expression of soluble antibody. Methods: P3-G9 phage antibody expression vector was reconstructed by DNA recombination method. The expression of soluble Fab in P3G9-L was identified by Western blotting. Results: 1.P3G9-L is a single Lac promoter that drives the expression of soluble Fab fragment of bicistronic Fd fragment and K strand simultaneously. The role of the Lac promoter in P3G9-L is modest, and the level of soluble Fab is low. Conclusions: 1 Lac is initiated by an expression system that can be used for soluble antibody Fabs. The double Lac promoter can be completely replaced by a single Lac promoter