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用从烟草里克隆的ＴＡ２９启动子分离自Ｂａｃｉｌｕｓａｍｙｌｏｌｉｑｕｅｆａｃｉｅｎｓ的核糖核酸酶基因ｂａｒｎａｓｅ，构建成雄性不育嵌合基因。再用农杆菌介导法转化番茄子叶，获得了具有雄性不育特征的转基因植株。
The ribonuclease gene barnase isolated from Bacillus subtilisfaciens was isolated from the TA29 promoter cloned from tobacco and constructed into a male sterile chimeric gene. Then the tomato cotyledons were transformed by Agrobacterium tumefaciens, and the transgenic plants with male sterility characteristics were obtained.