提出用分光光度法测定药物N-羟甲基烟酰胺的方法,N-羟甲基烟酰胺在酸催化下用醛酐转化为相应的醋酸酯,接着在碱催化下进行醋酸酯的羟肟化,最后用三氯化铁形成红色络合物溶液,该红色溶液对波长524nm有一最大吸收峰,实验表明当N-羟甲基烟酰胺的浓度为1～6×10~(-3)mol/l时能很好地遵守Beer定律。 A method for the spectrophotometric determination of the drug N-methylolnicotinamide is proposed. N-methylol nicotinamide is converted to the corresponding acetate using an aldehyde anhydride under acid catalysis, followed by hydroximation of the acetate under base catalysis , And finally with ferric chloride to form a red complex solution which has a maximum absorption peak at a wavelength of 524 nm. Experiments show that when the concentration of N-methylol nicotinamide is 1 to 6 × 10 -3 mol / Beer law can be well observed.