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目的探索流式细胞术用于细菌药敏试验的可行性。方法以碘化丙啶(PI)为荧光染料,用流式细胞术(FCM)检测对头孢噻肟敏感性不同的大肠埃希菌在不同浓度和不同时间头孢噻肟作用下各管平均荧光强度(MFI),并将FCM检测结果与菌落计数进行比较。结果细菌MFI值随着抗生素浓度的增加和作用时间的延长而增强,而菌落计数却随之减少,其变化具有时间依赖性和浓度依赖性,两者具有较好的一致性。整个试验只需2h。结论流式细胞术具有快速、敏感的检测药物对细菌的抗菌效应,该方法在细菌药敏试验中的应用,值得我们做进一步研究。
Objective To explore the feasibility of flow cytometry in bacterial susceptibility testing. Methods Propidium iodide (PI) was used as fluorescent dye to detect the average fluorescence intensity of each tube of cefotiam with different sensitivity to cefotaxime by flow cytometry (FCM) at different concentrations and different times of cefotaxime (MFI), and FCM test results were compared with the colony count. Results Bacterial MFI increased with the increase of antibiotic concentration and prolongation of action time, but the colony count decreased. The change of the MFI was time-dependent and concentration-dependent, and the two had good consistency. The whole experiment only 2h. Conclusion Flow cytometry has a fast and sensitive detection of antibacterial effect of drugs on bacteria. The application of this method in bacterial susceptibility testing deserves our further study.