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逆相蒸发法(REV)是制备单层脂质体的方法,有较高的包裹率。近年来,改良的REV可获得两种多层脂质体:MLV-REV脂粒和SPLV脂粒。MLV-REV脂粒的包裹率近65%,SPLV脂粒的包裹率为35～40%。本文的目的是进一步提高MLV-REV法的包裹率,并探讨其脂粒结构特征,与SPLV法制成的多层脂粒进行比较。 Small等曾比较平面双分子层包裹的水容积和脂质体包裹的水容积,以此来阐明脂质体的结构。作者用X-线衍射测量,表明MLV-REV和SPLV脂粒中的长空间(long spacing)与平面双分子层的长空间相似。因
Reverse Phase Evaporation (REV) is a method for preparing monolayer liposomes with a high rate of entrapment. In recent years, two types of multilamellar liposomes have been available for improved REV: MLV-REV lipid and SPLV lipid. The encapsulation rate of MLV-REV fat particles is nearly 65%, and the encapsulation rate of SPLV fat particles is 35 to 40%. The purpose of this paper is to further improve the parcel rate of MLV-REV method and to explore the characteristics of its lipid structure, compared with the multilayer fat particles made by SPLV method. Small et al. Have compared the volume of water contained in the planar bilayer with the water volume encapsulated by the liposome in order to elucidate the structure of the liposome. The authors measured with X-ray diffraction, indicating that the long spacing in the MLV-REV and SPLV lipids is similar to that of the planar bilayer. because