本文报告用高效液相色谱法同时测定血浆中布比卡因、利多卡因浓度。方法的线性范围分别为0.2～8μg/ml(布比卡因)和0.4～10μg/ml(利多卡因);r 值分别为0.9974和0.9900;检测限分别为0.06和0.03μg/ml;CV%一般均小于10%。并进行了方法的专一性试验。应用本法对布比卡因硬膜外阻滞后的血药浓度进行了监测,平均高峰浓度为1.73±0.70μg/ml,测得最高浓度为3.21μg/ml,未见不良反应发生。 This paper reports the simultaneous determination of bupivacaine and lidocaine in plasma by high performance liquid chromatography. The linear range of the method was 0.2 ~ 8μg / ml (bupivacaine) and 0.4 ~ 10μg / ml (lidocaine) respectively. The r values ​​were 0.9974 and 0.9900 respectively. The limits of detection were 0.06 and 0.03μg / ml, Generally less than 10%. And carried out a method of specificity test. This method was used to monitor the concentration of bupivacaine after epidural blockade, the average peak concentration of 1.73 ± 0.70μg / ml, the highest concentration was 3.21μg / ml, no adverse reactions.