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从形态性状上看,银榆杨是银白杨×白榆的科间杂种.为了进一步从分子水平上搞清楚银榆杨与其亲本银白杨及白榆的亲缘关系,该研究采用AFLP技术,用20对EcoRⅠ+MseⅠ引物对银榆杨、银白杨、白榆等10个样本进行了亲子关系分析.共获得2 040条可统计的谱带,其中1 470条带为多态性带,多态带百分率为72.06%.结果表明:①银榆杨中既含有银白杨的基因又含有白榆的基因,且出现了双亲不具有的新谱带;②银榆杨含有的银白杨基因成分比白榆基因成分多,从聚类分析(UPGMA)结果看出,银榆杨属于偏母本型的杂种;③辽宁产地的银白杨是银榆杨杂种的母本得到进一步证实.在所有白榆样本中,尽管方差分析结果表明各无性系间对银榆杨杂种子代的遗传距离没有显著差异,但辽宁产地的白榆2号(LP-2)与银榆杨杂种各无性系的遗传距离最小,它是银榆杨父本的可能性最大;④银榆杨中白榆的基因在4个白榆样本中的存在具有普遍性.但相对于辽宁产地的白榆而言,北京的白榆与银榆杨杂种的亲缘关系较远.该文还对银榆杨杂种的形成与遗传组成进行了分析讨论.
In terms of morphological traits, YinYuYin is interracial hybrid between Populus × white elm.In order to further clarify the genetic relationship between YinYuang and its parents, Populus alba and Ulmus pumila, at molecular level, this study used AFLP technique with 20 The paternity analysis of EcoRⅠ + MseⅠ primer pairs showed that there were 2040 statistical bands, of which 1 470 bands were polymorphic bands and polymorphic bands The percentage was 72.06%. The results showed that: ① Yin Yin contains both the gene of P. alba and the gene of P. elm, and there is a new band that the parents did not have; ② Yin Yin The result of cluster analysis (UPGMA) showed that P. yunnanensis belongs to the partial mother-type hybrids. (3) The female parent of Populus tomentosa was further confirmed in Liaoning Province, Although the results of ANOVA showed that there was no significant difference in the genetic distance between the elite progenies of silver elm in clones, the genetic distance between LP-2 and silver elm hybrid clones in Liaoning origin was the smallest, It is the most likely parent of silver elm Yang; silver elm Yang white elm Of the four elm samples in the presence of universal, but compared to the elm in Liaoning origin, Beijing elm and Silver Elm hybrids distantly related.This article also Yinyin hybrids Formation and genetic composition were analyzed and discussed.