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本文通过牛皮胶原与生物素-N-羟基琥珀酰亚胺(BNHS)共价键结合使胶原生物素化,再通过生物素-卵白素之间的生化反应使药理活性分子专一性地结合在胶原上。生物素化的胶原与卵白素的结合活性用一种体外的斑点试验定性分析,并用光学数字显示仪可实现定量数据处理。这里采用了两种结合程序:(1)用辣根过氧化物酶标记的卵白素与生物素化的胶原反应(LAB法)。通过辣根过氧化酶的显色作用检测卵白素与生物素胶原的结合率。(2)用游离的卵白素与两种生物素化的基
In this paper, collagen is biotinylated by the covalent bonding of cow skin collagen with biotin-N-hydroxysuccinimide (BNHS), and the pharmacologically active molecules are specifically bound by the biochemical reaction between biotin-avidin Collagen. The binding activity of biotinylated collagen to avidin is qualitatively analyzed with an in vitro speckle assay and quantified with an optical digital display. Two binding procedures are used here: (1) Horseradish peroxidase-labeled avidin reacts with biotinylated collagen (LAB method). The binding of avidin to biotinylated collagen was detected by the color development of horseradish peroxidase. (2) with free avidin and two biotinylated bases