论文部分内容阅读
本文在前期工作基础上,研究了两种海参肠自溶寡肽Val-Gly-Thr-Val-Glu-Met、Val-Thr-Pro-Tyr的抗氧化活性。应用电子自旋共振的方法(ESR),检测海参肠自溶寡肽对羟基自由基(·OH)、超氧阴离子自由基(O2-·)、1,1-二苯基-2-三硝基苯肼(DPPH·)自由基的清除情况。同时,采用人白血病细胞Jurkat,考察海参肠自溶寡肽对H2O2诱导的细胞氧化损伤的影响。结果表明,两种海参肠自溶寡肽对·OH、O2-·和DPPH自由基均有很强的清除能力,且呈明显的量效关系。对于不同自由基,Val-Gly-Thr-Val-Glu-Met在相应的浓度下(·OH自由基-1和5 mM、O2-·自由基-1、5和20 mM、DPPH自由基-1和8 mM)清除能力均显著高于Val-Thr-Pro-Tyr(p<0.05)。海参肠自溶寡肽在浓度为5 mM时未能对H2O2诱导的Jurkat细胞DNA氧化损伤起到保护作用,而浓度为20 mM时引起Jurkat细胞死亡。这些结果说明,海参肠自溶寡肽Val-Gly-Thr-Val-Glu-Met和Val-Thr-Pro-Tyr具有自由基清除能力,具有一定的开发价值。
Based on the previous work, we studied the antioxidant activity of Val-Gly-Thr-Val-Glu-Met and Val-Thr-Pro-Tyr. The effects of sea cucumber intestinal autolysis oligopeptide on the hydroxyl radical (· OH), superoxide anion radical (O2- ·), 1,1-diphenyl-2-trinitrate Cleavage of phenylhydrazine (DPPH ·) free radicals. At the same time, human leukemia cell Jurkat was used to investigate the effect of sea cucumber intestinal autolysis oligopeptide on H2O2-induced cellular oxidative damage. The results showed that the two kinds of sea cucumber intestinal autolysed oligopeptides have strong scavenging ability on · OH, O2- · and DPPH free radicals, and showed a significant dose-effect relationship. Val-Gly-Thr-Val-Glu-Met at different concentrations (· OH radicals -1 and 5 mM, O2- · Radicals -1, 5 and 20 mM, DPPH Radical -1 And 8 mM) were significantly higher than Val-Thr-Pro-Tyr (p <0.05). Sea cucumber intestinal autolytic oligopeptide failed to protect H2O2-induced DNA oxidative damage in Jurkat cells at a concentration of 5 mM, whereas Jurkat cell death was induced at a concentration of 20 mM. These results suggest that Val-Gly-Thr-Val-Glu-Met and Val-Thr-Pro-Tyr have free radical scavenging activity and have certain developmental value.