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目的研究养血补肝药物何首乌的抗抑郁作用和机制。方法采用束缚联合慢性不可预知温和应激大鼠、小鼠抑郁模型,通过敞箱行为和蔗糖水偏嗜度测试评价何首乌的抗抑郁药效作用。通过RT-PCR技术分析海马5HT1A基因表达,通过腹腔注射溴脱氧核苷尿嘧啶(Brdu)标记新生神经细胞,并以酶联免疫(ELISA)检测分析脑组织神经细胞发生。结果何首乌(2 g/kg)增加了慢性应激大鼠的水平运动、蔗糖水偏嗜度(P<0.05),以及海马5HT1AmRNA表达(P<0.01)。何首乌(2.85 g/kg)增加了慢性应激小鼠脑组织中Brdu含量(P<0.01),以上作用与氟西汀相似。结论何首乌可以改善慢性应激动物的抑郁行为,其机制可能与改善5HT能神经元传递,增加中枢神经细胞增殖等有关。
Objective To study the antidepressant effect and mechanism of nourishing blood and supplementing liver medicine Polygonum multiflorum. Methods The depression model of rats and mice with mild and unpredictable mild unpredictable stress was used to evaluate the antidepressant effect of Polygonum multiflorum Thunb. The expression of 5HT1A gene in the hippocampus was analyzed by RT-PCR, and the neonate neurons were labeled by intraperitoneal injection of bromodeoxyuridine (Brdu). The neuronal cells were detected by enzyme-linked immunosorbent assay (ELISA). Results Polygoni multiflori (2 g / kg) increased the level of exercise, sucrose water bias (P <0.05), and the expression of 5HT1A mRNA in the hippocampus of chronic stress rats (P <0.01). Polygonum multiflorum (2.85 g / kg) increased Brdu content in the brain of chronic stress mice (P <0.01), similar to fluoxetine. Conclusion Polygonum multiflorum can improve the depressive behavior of chronic stress animal. Its mechanism may be related to improving the transmission of 5HT neurons and increasing the proliferation of central nervous cells.