产毒性大肠杆菌产生两种肠毒素,其检测方法如下: 一、耐热肠毒素(ST) ST的检测较困难,这是由于sT是分子量较小的多肽,不具有抗原性,因此一般的免疫学检验方法受到了限制,目前仍以动物实验为主,尤以乳鼠灌喂实验为依据, 由于忽视了培养方法和操作条件,阳性检出率不高,重复性也差。作者创用L形管培养法获得十分满意的结果,比国内常用的 Toxigenic Escherichia coli produces two enterotoxins, the detection method is as follows: First, heat-resistant enterotoxin (ST) ST detection more difficult, because sT is a smaller molecular weight polypeptide, not antigenic, so the general immunity The test method is limited. At present, animal experiments are still the main method. In particular, based on the experiment of sucking mice, neglecting the culture method and operating conditions, the positive detection rate is low and the repeatability is poor. The author of the L-tube culture method to obtain very satisfied results, more commonly used than the domestic