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异硫氰酸荧光素(FITC)具有很强的免疫原性, 很容易产生与半抗原结合的特异抗体。作者报告了抗FITC MoAbs的制备及应用~(125)I-抗FITC MoAb测定不同抗原和抗体的实验结果。抗FITC MoAbs的制备:①免疫:将FITC-蜗牛血蓝蛋白100μg加福氏完全佐剂腹腔注入BALB/C小鼠,三个月后用等量的无佐剂抗原静脉注射。(2)融合:加强免疫4天后,把免疫小鼠的脾细胞和小鼠骨髓瘤细胞×63-Ag8.653一是通过改良的PEG法
Fluorescein isothiocyanate (FITC) has strong immunogenicity, it is easy to produce specific antibodies and hapten. The authors report the preparation of anti-FITC MoAbs and the experimental results using ~ (125) I-anti-FITC MoAbs for the determination of different antigens and antibodies. Preparation of Anti-FITC MoAbs: ① Immunization: BALB / C mice were injected intraperitoneally with 100 μg of FITC-snail hemocyanin and complete Freund’s adjuvant. After three months, mice were injected intravenously with an equal amount of unadjuvanted antigen. (2) Fusion: Four days after booster immunization, the spleen cells of immunized mice and the myeloma cells of mouse × 63-Ag8.653 were treated with modified PEG method