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目的 探讨钙调素拮抗剂三氟拉嗪（ＴＦＰ）对ＨｅＬａ细胞Ｓ期进程的影响及其分子机理。 方法 通过ＴｄＲ双阻断法获得同步化的Ｓ期ＨｅＬａ细胞，以３Ｈ－ＴｄＲ掺入法和Ｗｅｓｔｅｒｎ 技术分别观察了ＴＦＰ对ＨｅＬａ 细胞Ｓ期进程和胸苷激酶（ｔｈｙｍ ｉｄｉｎｅ ｋｉｎａｓｅ，ＴＫ）活性及细胞周期引擎分子ＣｙｃｌｉｎＡ、Ｃｄｋ２ 和细胞周期调节蛋白ｐ８０ｃｄｃ２５Ｂ、ＰＣＮＡ、ｐ２１ 蛋白表达水平的影响。 结果 ＴＦＰ（２０μｍ ｏｌ／Ｌ）能使３ Ｈ－ＴｄＲ的掺入峰值后移，并抑制了Ｃｙ－ｃｌｉｎＡ、ＰＣＮＡ、ｐ８０ｃｄｃ２５Ｂ的表达和提高了ｐ２１ 蛋白的水平，但对Ｃｄｋ２的表达几乎无影响。 结论 ＣａＭ 除了能通过影响ＰＣＮＡ的水平直接作用于ＤＮＡ 合成，同时又可通过作用于ＣｙｃｌｉｎＡ、ｐ８０ｃｄｃ２５Ｂ、ｐ２１ 等周期引擎分子和调控因子水平正调ＨｅＬａ 细胞Ｓ期进程。
Objective To investigate the effect of calmodulin antagonist trifluoperazine (TFP) on the S phase of HeLa cells and its molecular mechanism. Methods The synchronized S-phase HeLa cells were obtained by TdR double-block method. The effect of TFP on the S phase and the activity of thymidine kinase (TK) and the activity of TFP in HeLa cells were observed by 3H-TdR incorporation and Western blot respectively. CyclinA, Cdk2 and the expression of cell cycle regulatory proteins p80cdc25B, PCNA, p21 in the engineered cyclone. Results TFP (20μmol / L) could reverse the incorporation peak of 3 H-TdR, inhibit the expression of Cy-clinA, PCNA and p80cdc25B and increase the level of p21 protein, but had little effect on the expression of Cdk2. Conclusion CaM can act directly on DNA synthesis by affecting the level of PCNA, and up-regulate the S-phase of HeLa cells by acting on the expression of cyclin A, p80cdc25B, p21 and other cyclical molecular and regulators.